Emma Lamb has withdrawn from England's squad for the one-off Test match against India with a back injury.
Opening batter Lamb, 25, has returned to the UK to see a spinal surgeon.
Batter Maia Bouchier and spinner Kirstie Gordon have been called up to the squad for the four-day Test that starts in Mumbai on 14 December.
Bouchier is uncapped in Test cricket, while Gordon played one match against Australia in 2019 but has not represented England since.
Bouchier, 25, is part of Heather Knight's squad for the three-match T20 series which precedes the Test, but did not feature in England's opening 38-run win.
Gordon, 26, played for England A in their recent 2-1 T20 series victory over India A, and has remained in India with the full squad since.
Lamb's absence as a specialist opener will likely mean one of Danni Wyatt, Sophia Dunkley or Alice Capsey will join Tammy Beaumont at the top of the order, with all three having fulfilled the role in white-ball cricket.
The final two T20s take place on Saturday and Sunday at the Wankhede Stadium before the Test match moves to the DY Patil Stadium, both in Mumbai.
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